Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 114519..115162 Replicon plasmid pE275a_123
Accession NZ_CP035866
Organism Escherichia coli strain E275 a

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag EXE34_RS25250 Protein ID WP_001044768.1
Coordinates 114519..114935 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID E9XUD3
Locus tag EXE34_RS25255 Protein ID WP_001261279.1
Coordinates 114932..115162 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE34_RS25225 110382..111581 + 1200 Protein_120 IS3 family transposase -
EXE34_RS25230 111785..112351 + 567 WP_000587873.1 hypothetical protein -
EXE34_RS25235 112419..112604 - 186 Protein_122 transposase domain-containing protein -
EXE34_RS25240 112674..112817 + 144 Protein_123 transposase -
EXE34_RS25245 113188..113796 - 609 WP_000515889.1 ProQ/FINO family protein -
EXE34_RS25250 114519..114935 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
EXE34_RS25255 114932..115162 - 231 WP_001261279.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EXE34_RS25260 115766..116179 + 414 WP_000465051.1 hypothetical protein -
EXE34_RS25265 116181..116966 + 786 WP_001164197.1 site-specific integrase -
EXE34_RS25270 117550..118182 + 633 WP_000312331.1 ParA family protein -
EXE34_RS25275 118182..118556 + 375 WP_000752652.1 hypothetical protein -
EXE34_RS25280 118795..119769 + 975 WP_001217836.1 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA15 / cmlA1 / aadA2 / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A - 1..123312 123312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T118900 WP_001044768.1 NZ_CP035866:c114935-114519 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T118900 NZ_CP035866:c114935-114519 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8761.90 Da        Isoelectric Point: 4.6310

>AT118900 WP_001261279.1 NZ_CP035866:c115162-114932 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPVRPAWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT118900 NZ_CP035866:c115162-114932 [Escherichia coli]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAAGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGCCCGGCCTGGGGCTCGTTCGCGCAGCTCG
AGAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A3U0EQM4

References