Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 916822..917440 Replicon chromosome
Accession NZ_CP035865
Organism Escherichia coli strain E275 a

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXE34_RS04435 Protein ID WP_001291435.1
Coordinates 916822..917040 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXE34_RS04440 Protein ID WP_000344800.1
Coordinates 917066..917440 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE34_RS04400 (912111) 912111..912683 + 573 WP_000779831.1 YbaY family lipoprotein -
EXE34_RS04405 (912714) 912714..913025 - 312 WP_000409911.1 MGMT family protein -
EXE34_RS04415 (913404) 913404..913757 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EXE34_RS04420 (913799) 913799..915349 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXE34_RS04425 (915513) 915513..915983 - 471 WP_000136192.1 YlaC family protein -
EXE34_RS04430 (916099) 916099..916650 - 552 WP_000102564.1 maltose O-acetyltransferase -
EXE34_RS04435 (916822) 916822..917040 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EXE34_RS04440 (917066) 917066..917440 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXE34_RS04445 (917986) 917986..921135 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXE34_RS04450 (921158) 921158..922351 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118881 WP_001291435.1 NZ_CP035865:c917040-916822 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118881 NZ_CP035865:c917040-916822 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118881 WP_000344800.1 NZ_CP035865:c917440-917066 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118881 NZ_CP035865:c917440-917066 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References