Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4433718..4434215 Replicon chromosome
Accession NZ_CP035860
Organism Escherichia coli strain TD225-C4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EXA00_RS21880 Protein ID WP_001173343.1
Coordinates 4433718..4433891 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag EXA00_RS21885 Protein ID WP_001425728.1
Coordinates 4433919..4434215 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA00_RS21870 (4430388) 4430388..4431845 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
EXA00_RS21875 (4432082) 4432082..4433599 + 1518 WP_001295074.1 lysine--tRNA ligase -
EXA00_RS21880 (4433718) 4433718..4433891 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EXA00_RS21885 (4433919) 4433919..4434215 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EXA00_RS21890 (4434442) 4434442..4434714 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EXA00_RS21895 (4434726) 4434726..4434956 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
EXA00_RS21900 (4435137) 4435137..4436768 + 1632 WP_001216487.1 sensor histidine kinase -
EXA00_RS21905 (4436765) 4436765..4437484 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118873 WP_001173343.1 NZ_CP035860:c4433891-4433718 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118873 NZ_CP035860:c4433891-4433718 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT118873 WP_001425728.1 NZ_CP035860:c4434215-4433919 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118873 NZ_CP035860:c4434215-4433919 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References