Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3086015..3086486 Replicon chromosome
Accession NZ_CP035860
Organism Escherichia coli strain TD225-C4

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag EXA00_RS15380 Protein ID WP_001303511.1
Coordinates 3086208..3086486 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EXA00_RS15375 Protein ID WP_001302048.1
Coordinates 3086015..3086206 (+) Length 64 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA00_RS15335 (3081463) 3081463..3081942 + 480 WP_000715488.1 hypothetical protein -
EXA00_RS15340 (3081948) 3081948..3082472 - 525 WP_235834910.1 antiterminator Q family protein -
EXA00_RS15345 (3082631) 3082631..3083047 - 417 WP_032201964.1 DUF977 family protein -
EXA00_RS15350 (3083062) 3083062..3083808 - 747 WP_000788772.1 ATP-binding protein -
EXA00_RS15355 (3083815) 3083815..3084780 - 966 WP_169029974.1 hypothetical protein -
EXA00_RS15360 (3084801) 3084801..3085226 - 426 WP_032201966.1 toxin YdaT family protein -
EXA00_RS15365 (3085223) 3085223..3085525 - 303 WP_000172738.1 transcriptional regulator -
EXA00_RS15370 (3085623) 3085623..3085994 + 372 WP_001169687.1 hypothetical protein -
EXA00_RS15375 (3086015) 3086015..3086206 + 192 WP_001302048.1 hypothetical protein Antitoxin
EXA00_RS15380 (3086208) 3086208..3086486 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EXA00_RS15385 (3086774) 3086774..3086929 + 156 WP_000379557.1 DUF1391 family protein -
EXA00_RS15390 (3087089) 3087089..3087307 + 219 WP_001171921.1 protein YdfC -
EXA00_RS15395 (3087330) 3087330..3087737 + 408 WP_000394517.1 hypothetical protein -
EXA00_RS15400 (3087715) 3087715..3087948 - 234 WP_001735122.1 hypothetical protein -
EXA00_RS15405 (3088479) 3088479..3088667 + 189 WP_000449197.1 cell division inhibition protein DicB -
EXA00_RS15410 (3088664) 3088664..3088867 + 204 WP_001098304.1 DUF1482 family protein -
EXA00_RS15415 (3088948) 3088948..3091419 + 2472 WP_216334070.1 exonuclease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-91)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T118871 WP_001303511.1 NZ_CP035860:3086208-3086486 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T118871 NZ_CP035860:3086208-3086486 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT118871 WP_001302048.1 NZ_CP035860:3086015-3086206 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT118871 NZ_CP035860:3086015-3086206 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE


Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References