Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 332668..333254 Replicon chromosome
Accession NZ_CP035860
Organism Escherichia coli strain TD225-C4

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag EXA00_RS01530 Protein ID WP_042014162.1
Coordinates 332668..332898 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EXA00_RS01535 Protein ID WP_000593555.1
Coordinates 332895..333254 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA00_RS01520 (328705) 328705..331440 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
EXA00_RS01525 (331440) 331440..332564 + 1125 WP_001314210.1 ABC transporter permease -
EXA00_RS01530 (332668) 332668..332898 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
EXA00_RS01535 (332895) 332895..333254 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EXA00_RS01540 (333374) 333374..333775 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
EXA00_RS01545 (333781) 333781..334587 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
EXA00_RS01550 (334584) 334584..335348 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
EXA00_RS01555 (335348) 335348..336181 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
EXA00_RS01560 (336178) 336178..337122 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T118852 WP_042014162.1 NZ_CP035860:332668-332898 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T118852 NZ_CP035860:332668-332898 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT118852 WP_000593555.1 NZ_CP035860:332895-333254 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT118852 NZ_CP035860:332895-333254 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References