Detailed information of TA system TA118851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 51244..51803
• Replicon: plasmid pTW11681_74
• Accession: NZ_CP035859
• Organism: Escherichia coli TW11681

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EXE31_RS25775
• Protein ID: WP_000421261.1
• Coordinates: 51528..51803 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EXE31_RS25770
• Protein ID: WP_001297026.1
• Coordinates: 51244..51528 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE31_RS26215 (46654)	46654..46800	-	147	WP_235777688.1	PilI type IV pilus biogenesis protein	-
EXE31_RS25755 (47155)	47155..47820	-	666	WP_000154140.1	hypothetical protein	-
EXE31_RS25760 (47961)	47961..48467	-	507	WP_001317510.1	transcription termination/antitermination NusG family protein	-
EXE31_RS25765 (49305)	49305..50336	+	1032	WP_000907882.1	plasmid replication initiator RepA	-
EXE31_RS25770 (51244)	51244..51528	+	285	WP_001297026.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXE31_RS25775 (51528)	51528..51803	+	276	WP_000421261.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXE31_RS25780 (51909)	51909..52112	+	204	Protein_55	hypothetical protein	-
EXE31_RS25785 (52381)	52381..52647	-	267	WP_000627831.1	hypothetical protein	-
EXE31_RS25790 (52785)	52785..53387	-	603	WP_000517694.1	ProQ/FINO family protein	-
EXE31_RS25795 (53741)	53741..55087	+	1347	WP_001132022.1	protein YagA	-
EXE31_RS25800 (55598)	55598..55900	-	303	WP_001245884.1	hypothetical protein	-
EXE31_RS25805 (55897)	55897..56523	-	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..74310	74310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T118851 WP_000421261.1 NZ_CP035859:51528-51803 [Escherichia coli TW11681]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEVRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118851 NZ_CP035859:51528-51803 [Escherichia coli TW11681]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAGTCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10502.95 Da
• Isoelectric Point: 5.6558

>AT118851 WP_001297026.1 NZ_CP035859:51244-51528 [Escherichia coli TW11681]
MQMKNNTVQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118851 NZ_CP035859:51244-51528 [Escherichia coli TW11681]
ATGCAAATGAAAAACAATACCGTACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA