Detailed information of TA system TA118846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 26845..27470
• Replicon: plasmid pTW11681_81
• Accession: NZ_CP035858
• Organism: Escherichia coli TW11681

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXE31_RS25195
• Protein ID: WP_000911313.1
• Coordinates: 26845..27243 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: EXE31_RS25200
• Protein ID: WP_000450520.1
• Coordinates: 27243..27470 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE31_RS25175 (22156)	22156..22833	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
EXE31_RS25180 (22833)	22833..23180	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
EXE31_RS25185 (23200)	23200..24771	+	1572	WP_001748001.1	IS66-like element ISCro1 family transposase	-
EXE31_RS25190 (24803)	24803..26836	+	2034	Protein_29	type IV conjugative transfer system coupling protein TraD	-
EXE31_RS25195 (26845)	26845..27243	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXE31_RS25200 (27243)	27243..27470	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..81910	81910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T118846 WP_000911313.1 NZ_CP035858:c27243-26845 [Escherichia coli TW11681]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T118846 NZ_CP035858:c27243-26845 [Escherichia coli TW11681]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT118846 WP_000450520.1 NZ_CP035858:c27470-27243 [Escherichia coli TW11681]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT118846 NZ_CP035858:c27470-27243 [Escherichia coli TW11681]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2