Detailed information of TA system TA118845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 77682..78241
• Replicon: plasmid pTW11681_100
• Accession: NZ_CP035856
• Organism: Escherichia coli TW11681

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: EXE31_RS24335
• Protein ID: WP_000421265.1
• Coordinates: 77966..78241 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: EXE31_RS24330
• Protein ID: WP_001743519.1
• Coordinates: 77682..77966 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE31_RS24315	73869..74435	+	567	WP_000904908.1	recombinase family protein	-
EXE31_RS26145	74432..74845	+	414	WP_001046769.1	hypothetical protein	-
EXE31_RS24325	75177..76928	+	1752	WP_000038721.1	ATP-binding protein	-
EXE31_RS24330	77682..77966	+	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXE31_RS24335	77966..78241	+	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXE31_RS24340	78404..79480	+	1077	WP_000610004.1	hypothetical protein	-
EXE31_RS24345	79731..79934	+	204	Protein_82	hypothetical protein	-
EXE31_RS24355	80543..80617	-	75	Protein_83	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EXE31_RS24360	82031..82426	-	396	WP_000445942.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaE / cfaC / cfaB / cfaA / etpB / etpA / cfaD'	1..100284	100284
	-	flank	IS/Tn	-	-	70690..73824	3134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T118845 WP_000421265.1 NZ_CP035856:77966-78241 [Escherichia coli TW11681]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118845 NZ_CP035856:77966-78241 [Escherichia coli TW11681]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT118845 WP_001743519.1 NZ_CP035856:77682-77966 [Escherichia coli TW11681]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118845 NZ_CP035856:77682-77966 [Escherichia coli TW11681]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1