Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4920393..4921011 Replicon chromosome
Accession NZ_CP035855
Organism Escherichia coli TW11681

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXE31_RS23880 Protein ID WP_001291435.1
Coordinates 4920393..4920611 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXE31_RS23885 Protein ID WP_000344800.1
Coordinates 4920637..4921011 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE31_RS23845 (4915682) 4915682..4916254 + 573 WP_000779826.1 YbaY family lipoprotein -
EXE31_RS23850 (4916285) 4916285..4916596 - 312 WP_000409911.1 MGMT family protein -
EXE31_RS23860 (4916975) 4916975..4917328 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EXE31_RS23865 (4917370) 4917370..4918920 - 1551 WP_001484361.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXE31_RS23870 (4919084) 4919084..4919554 - 471 WP_000136192.1 YlaC family protein -
EXE31_RS23875 (4919670) 4919670..4920221 - 552 WP_000102568.1 maltose O-acetyltransferase -
EXE31_RS23880 (4920393) 4920393..4920611 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EXE31_RS23885 (4920637) 4920637..4921011 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXE31_RS23890 (4921557) 4921557..4924706 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXE31_RS23895 (4924729) 4924729..4925922 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118844 WP_001291435.1 NZ_CP035855:c4920611-4920393 [Escherichia coli TW11681]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118844 NZ_CP035855:c4920611-4920393 [Escherichia coli TW11681]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118844 WP_000344800.1 NZ_CP035855:c4921011-4920637 [Escherichia coli TW11681]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118844 NZ_CP035855:c4921011-4920637 [Escherichia coli TW11681]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References