Detailed information of TA system TA118843

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4688964..4689643
• Replicon: chromosome
• Accession: NZ_CP035855
• Organism: Escherichia coli TW11681

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: EXE31_RS22725
• Protein ID: WP_000854680.1
• Coordinates: 4688964..4689305 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: EXE31_RS22730
• Protein ID: WP_000070396.1
• Coordinates: 4689326..4689643 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE31_RS22710 (4685043)	4685043..4685390	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
EXE31_RS22715 (4685390)	4685390..4686067	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
EXE31_RS22720 (4686763)	4686763..4687869	+	1107	WP_000633074.1	SMEK domain-containing protein	-
EXE31_RS22725 (4688964)	4688964..4689305	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
EXE31_RS22730 (4689326)	4689326..4689643	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
EXE31_RS22735 (4689662)	4689662..4689883	-	222	WP_000691994.1	DUF987 domain-containing protein	-
EXE31_RS22740 (4689892)	4689892..4690368	-	477	WP_000811693.1	RadC family protein	-
EXE31_RS22745 (4690384)	4690384..4690842	-	459	WP_000211838.1	antirestriction protein	-
EXE31_RS22750 (4690940)	4690940..4691179	-	240	WP_000194654.1	DUF905 family protein	-
EXE31_RS22755 (4691256)	4691256..4691723	-	468	WP_001385283.1	protein YkfB	-
EXE31_RS22760 (4691746)	4691746..4692189	-	444	WP_000649865.1	hypothetical protein	-
EXE31_RS22765 (4692189)	4692189..4692425	-	237	WP_001144031.1	protein YpjK	-
EXE31_RS22770 (4692466)	4692466..4693167	-	702	WP_000189411.1	WYL domain-containing protein	-
EXE31_RS22775 (4693384)	4693384..4694197	-	814	Protein_4451	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T118843 WP_000854680.1 NZ_CP035855:c4689305-4688964 [Escherichia coli TW11681]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T118843 NZ_CP035855:c4689305-4688964 [Escherichia coli TW11681]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT118843 WP_000070396.1 NZ_CP035855:c4689643-4689326 [Escherichia coli TW11681]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT118843 NZ_CP035855:c4689643-4689326 [Escherichia coli TW11681]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7