Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4071959..4072456 Replicon chromosome
Accession NZ_CP035855
Organism Escherichia coli TW11681

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EXE31_RS19750 Protein ID WP_001173343.1
Coordinates 4072283..4072456 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EXE31_RS19745 Protein ID WP_000398619.1
Coordinates 4071959..4072255 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE31_RS19725 (4068690) 4068690..4069409 - 720 WP_000611288.1 two-component system response regulator DcuR -
EXE31_RS19730 (4069406) 4069406..4071037 - 1632 WP_001216483.1 sensor histidine kinase -
EXE31_RS19735 (4071218) 4071218..4071448 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
EXE31_RS19740 (4071460) 4071460..4071732 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
EXE31_RS19745 (4071959) 4071959..4072255 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EXE31_RS19750 (4072283) 4072283..4072456 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EXE31_RS19755 (4072575) 4072575..4074092 - 1518 WP_001295089.1 lysine--tRNA ligase -
EXE31_RS19760 (4074329) 4074329..4075786 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118841 WP_001173343.1 NZ_CP035855:4072283-4072456 [Escherichia coli TW11681]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118841 NZ_CP035855:4072283-4072456 [Escherichia coli TW11681]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT118841 WP_000398619.1 NZ_CP035855:4071959-4072255 [Escherichia coli TW11681]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118841 NZ_CP035855:4071959-4072255 [Escherichia coli TW11681]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References