Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2655063..2655717 Replicon chromosome
Accession NZ_CP035855
Organism Escherichia coli TW11681

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EXE31_RS12940 Protein ID WP_000244781.1
Coordinates 2655063..2655470 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXE31_RS12945 Protein ID WP_000354046.1
Coordinates 2655451..2655717 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE31_RS12920 (2651020) 2651020..2652753 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
EXE31_RS12925 (2652759) 2652759..2653469 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXE31_RS12930 (2653494) 2653494..2654390 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXE31_RS12935 (2654502) 2654502..2655023 + 522 WP_001055874.1 flavodoxin FldB -
EXE31_RS12940 (2655063) 2655063..2655470 - 408 WP_000244781.1 protein YgfX Toxin
EXE31_RS12945 (2655451) 2655451..2655717 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXE31_RS12950 (2655960) 2655960..2656940 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
EXE31_RS12955 (2657142) 2657142..2657795 - 654 WP_000250273.1 hemolysin III family protein -
EXE31_RS12960 (2657959) 2657959..2658270 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EXE31_RS12965 (2658315) 2658315..2659748 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
EXE31_RS12970 (2659805) 2659805..2660548 - 744 WP_000951939.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T118838 WP_000244781.1 NZ_CP035855:c2655470-2655063 [Escherichia coli TW11681]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118838 NZ_CP035855:c2655470-2655063 [Escherichia coli TW11681]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118838 WP_000354046.1 NZ_CP035855:c2655717-2655451 [Escherichia coli TW11681]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118838 NZ_CP035855:c2655717-2655451 [Escherichia coli TW11681]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References