Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1061234..1061872 Replicon chromosome
Accession NZ_CP035855
Organism Escherichia coli TW11681

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EXE31_RS05275 Protein ID WP_000813794.1
Coordinates 1061234..1061410 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EXE31_RS05280 Protein ID WP_001270286.1
Coordinates 1061456..1061872 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE31_RS05255 (1056853) 1056853..1058028 - 1176 WP_001236305.1 BenE family transporter YdcO -
EXE31_RS05260 (1058120) 1058120..1058656 + 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
EXE31_RS05265 (1058729) 1058729..1060690 + 1962 WP_001295654.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EXE31_RS05270 (1060782) 1060782..1061012 - 231 WP_000494244.1 YncJ family protein -
EXE31_RS05275 (1061234) 1061234..1061410 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EXE31_RS05280 (1061456) 1061456..1061872 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EXE31_RS05285 (1061951) 1061951..1063357 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
EXE31_RS05290 (1063602) 1063602..1064747 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
EXE31_RS05295 (1064765) 1064765..1065778 + 1014 WP_000220382.1 ABC transporter ATP-binding protein -
EXE31_RS05300 (1065779) 1065779..1066720 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T118825 WP_000813794.1 NZ_CP035855:1061234-1061410 [Escherichia coli TW11681]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T118825 NZ_CP035855:1061234-1061410 [Escherichia coli TW11681]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT118825 WP_001270286.1 NZ_CP035855:1061456-1061872 [Escherichia coli TW11681]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT118825 NZ_CP035855:1061456-1061872 [Escherichia coli TW11681]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References