Detailed information of TA system TA118820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 11262..11821
• Replicon: plasmid pTW10590_71
• Accession: NZ_CP035854
• Organism: Escherichia coli strain TW10590

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EXE32_RS26180
• Protein ID: WP_000421261.1
• Coordinates: 11262..11537 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EXE32_RS26185
• Protein ID: WP_001297026.1
• Coordinates: 11537..11821 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE32_RS26150 (6534)	6534..7160	+	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-
EXE32_RS26155 (7157)	7157..7459	+	303	WP_001245884.1	hypothetical protein	-
EXE32_RS26160 (7978)	7978..9324	-	1347	WP_001132022.1	protein YagA	-
EXE32_RS26165 (9678)	9678..10280	+	603	WP_000517694.1	ProQ/FINO family protein	-
EXE32_RS26170 (10418)	10418..10684	+	267	WP_000627831.1	hypothetical protein	-
EXE32_RS26175 (10953)	10953..11156	-	204	Protein_15	hypothetical protein	-
EXE32_RS26180 (11262)	11262..11537	-	276	WP_000421261.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXE32_RS26185 (11537)	11537..11821	-	285	WP_001297026.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXE32_RS26190 (12729)	12729..13760	-	1032	WP_000907882.1	plasmid replication initiator RepA	-
EXE32_RS26195 (14463)	14463..15104	+	642	WP_000335851.1	transcription termination/antitermination NusG family protein	-
EXE32_RS26200 (15245)	15245..15910	+	666	WP_000154140.1	hypothetical protein	-
EXE32_RS26840 (16265)	16265..16411	+	147	WP_235777688.1	PilI type IV pilus biogenesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..71003	71003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T118820 WP_000421261.1 NZ_CP035854:c11537-11262 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEVRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118820 NZ_CP035854:c11537-11262 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAGTCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10502.95 Da
• Isoelectric Point: 5.6558

>AT118820 WP_001297026.1 NZ_CP035854:c11821-11537 [Escherichia coli]
MQMKNNTVQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118820 NZ_CP035854:c11821-11537 [Escherichia coli]
ATGCAAATGAAAAACAATACCGTACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA