Detailed information of TA system TA118819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 10850..11409
• Replicon: plasmid pTW10590_102
• Accession: NZ_CP035853
• Organism: Escherichia coli strain TW10590

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: EXE32_RS25615
• Protein ID: WP_000421265.1
• Coordinates: 10850..11125 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: EXE32_RS25620
• Protein ID: WP_001743519.1
• Coordinates: 11125..11409 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE32_RS25585	7138..7212	+	75	Protein_9	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EXE32_RS25595	7821..8024	-	204	Protein_10	hypothetical protein	-
EXE32_RS25600	8275..8973	-	699	WP_250186231.1	hypothetical protein	-
EXE32_RS25610	10352..10687	-	336	WP_250186236.1	hypothetical protein	-
EXE32_RS25615	10850..11125	-	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXE32_RS25620	11125..11409	-	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXE32_RS25625	12163..13914	-	1752	WP_126687970.1	ATP-binding protein	-
EXE32_RS26815	14246..14659	-	414	WP_001046769.1	hypothetical protein	-
EXE32_RS25635	14656..15222	-	567	WP_000904908.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaD' / cfaE / cfaC / cfaB / cfaA / etpA / etpB	1..102083	102083
	-	flank	IS/Tn	-	-	15294..18401	3107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T118819 WP_000421265.1 NZ_CP035853:c11125-10850 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118819 NZ_CP035853:c11125-10850 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT118819 WP_001743519.1 NZ_CP035853:c11409-11125 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118819 NZ_CP035853:c11409-11125 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1