Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 91530..92173 Replicon plasmid pTW10590_133
Accession NZ_CP035852
Organism Escherichia coli strain TW10590

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag EXE32_RS25270 Protein ID WP_001044768.1
Coordinates 91530..91946 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID E9XUD3
Locus tag EXE32_RS25275 Protein ID WP_001261279.1
Coordinates 91943..92173 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE32_RS25240 86653..87078 - 426 WP_000422741.1 transposase -
EXE32_RS25245 87443..88593 + 1151 Protein_83 IS3 family transposase -
EXE32_RS25250 88797..89363 + 567 WP_000587873.1 hypothetical protein -
EXE32_RS25255 89431..89640 - 210 Protein_85 transposase domain-containing protein -
EXE32_RS25260 89685..89828 + 144 Protein_86 transposase -
EXE32_RS25265 90199..90807 - 609 WP_000515889.1 ProQ/FINO family protein -
EXE32_RS25270 91530..91946 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
EXE32_RS25275 91943..92173 - 231 WP_001261279.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EXE32_RS25280 92778..93191 + 414 WP_001581782.1 hypothetical protein -
EXE32_RS25285 93193..93978 + 786 WP_001581781.1 site-specific integrase -
EXE32_RS25290 94562..95194 + 633 WP_000312331.1 ParA family protein -
EXE32_RS25295 95194..95568 + 375 WP_000752652.1 hypothetical protein -
EXE32_RS25300 95806..96780 + 975 WP_001217836.1 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - etpB / etpA 1..133955 133955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T118818 WP_001044768.1 NZ_CP035852:c91946-91530 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T118818 NZ_CP035852:c91946-91530 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8761.90 Da        Isoelectric Point: 4.6310

>AT118818 WP_001261279.1 NZ_CP035852:c92173-91943 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPVRPAWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT118818 NZ_CP035852:c92173-91943 [Escherichia coli]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAAGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGCCCGGCCTGGGGCTCGTTCGCGCAGCTCG
AGAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A3U0EQM4

References