Detailed information of TA system TA118801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2632982..2633607
• Replicon: chromosome
• Accession: NZ_CP035850
• Organism: Escherichia coli strain TW10590

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXE32_RS12570
• Protein ID: WP_000911330.1
• Coordinates: 2633209..2633607 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EXE32_RS12565
• Protein ID: WP_000450524.1
• Coordinates: 2632982..2633209 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE32_RS12540 (2628785)	2628785..2629255	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EXE32_RS12545 (2629255)	2629255..2629827	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EXE32_RS12550 (2629973)	2629973..2630851	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EXE32_RS12555 (2630868)	2630868..2631902	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EXE32_RS12560 (2632115)	2632115..2632828	+	714	WP_001437774.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EXE32_RS12565 (2632982)	2632982..2633209	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EXE32_RS12570 (2633209)	2633209..2633607	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXE32_RS12575 (2633754)	2633754..2634617	+	864	WP_001267485.1	neutral zinc metallopeptidase	-
EXE32_RS12580 (2634632)	2634632..2636647	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
EXE32_RS12585 (2636721)	2636721..2637419	+	699	WP_000679823.1	esterase	-
EXE32_RS12590 (2637529)	2637529..2637729	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T118801 WP_000911330.1 NZ_CP035850:2633209-2633607 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T118801 NZ_CP035850:2633209-2633607 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT118801 WP_000450524.1 NZ_CP035850:2632982-2633209 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT118801 NZ_CP035850:2632982-2633209 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCAGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33