Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 52607..53225 Replicon chromosome
Accession NZ_CP035850
Organism Escherichia coli strain TW10590

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXE32_RS00245 Protein ID WP_001291435.1
Coordinates 53007..53225 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXE32_RS00240 Protein ID WP_000344800.1
Coordinates 52607..52981 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE32_RS00230 (47696) 47696..48889 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EXE32_RS00235 (48912) 48912..52061 + 3150 WP_001132456.1 efflux RND transporter permease AcrB -
EXE32_RS00240 (52607) 52607..52981 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXE32_RS00245 (53007) 53007..53225 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EXE32_RS00250 (53396) 53396..53947 + 552 WP_000102578.1 maltose O-acetyltransferase -
EXE32_RS00255 (54063) 54063..54533 + 471 WP_000136192.1 YlaC family protein -
EXE32_RS00260 (54697) 54697..56247 + 1551 WP_126687887.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXE32_RS00265 (56289) 56289..56642 - 354 WP_000878141.1 DUF1428 family protein -
EXE32_RS00275 (57021) 57021..57332 + 312 WP_000409913.1 MGMT family protein -
EXE32_RS00280 (57363) 57363..57935 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118790 WP_001291435.1 NZ_CP035850:53007-53225 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118790 NZ_CP035850:53007-53225 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118790 WP_000344800.1 NZ_CP035850:52607-52981 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118790 NZ_CP035850:52607-52981 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References