Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 125380..125905 Replicon plasmid pTW10598_141
Accession NZ_CP035847
Organism Escherichia coli strain TW10598

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EXA01_RS24530 Protein ID WP_001159871.1
Coordinates 125600..125905 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EXA01_RS24525 Protein ID WP_000813634.1
Coordinates 125380..125598 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA01_RS24500 (121004) 121004..121954 - 951 WP_001528378.1 virulence factor VirK -
EXA01_RS24505 (121959) 121959..123047 - 1089 WP_001528375.1 putative hexosyltransferase CapU -
EXA01_RS24510 (123050) 123050..123892 - 843 WP_162749921.1 polysaccharide deacetylase family protein -
EXA01_RS24515 (124136) 124136..124678 - 543 WP_000194543.1 hypothetical protein -
EXA01_RS24520 (124678) 124678..124935 - 258 WP_000343093.1 hypothetical protein -
EXA01_RS24525 (125380) 125380..125598 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EXA01_RS24530 (125600) 125600..125905 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EXA01_RS24535 (125930) 125930..126619 + 690 Protein_163 site-specific integrase -
EXA01_RS24540 (126949) 126949..127590 + 642 WP_000470631.1 recombinase family protein -
EXA01_RS24545 (128030) 128030..128710 - 681 WP_000452091.1 EAL domain-containing protein -
EXA01_RS24550 (128958) 128958..129661 + 704 Protein_166 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - cs3 / etpB / eltA / eltB 1..141411 141411
- flank IS/Tn - - 126949..127590 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T118788 WP_001159871.1 NZ_CP035847:125600-125905 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T118788 NZ_CP035847:125600-125905 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT118788 WP_000813634.1 NZ_CP035847:125380-125598 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT118788 NZ_CP035847:125380-125598 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References