Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 27791..28316 Replicon plasmid pTW10598_141
Accession NZ_CP035847
Organism Escherichia coli strain TW10598

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EXA01_RS23935 Protein ID WP_001159871.1
Coordinates 28011..28316 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D7GK94
Locus tag EXA01_RS23930 Protein ID WP_000829078.1
Coordinates 27791..28009 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA01_RS23905 (23338) 23338..24288 - 951 WP_086405437.1 virulence factor VirK -
EXA01_RS23910 (24293) 24293..25381 - 1089 WP_001528375.1 putative hexosyltransferase CapU -
EXA01_RS23915 (25384) 25384..26226 - 843 WP_162749921.1 polysaccharide deacetylase family protein -
EXA01_RS23920 (26470) 26470..27012 - 543 WP_000194544.1 hypothetical protein -
EXA01_RS23925 (27012) 27012..27269 - 258 WP_000343095.1 hypothetical protein -
EXA01_RS23930 (27791) 27791..28009 + 219 WP_000829078.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EXA01_RS23935 (28011) 28011..28316 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EXA01_RS23940 (28317) 28317..29123 + 807 WP_000016960.1 site-specific integrase -
EXA01_RS23945 (29301) 29301..29945 + 645 WP_001144037.1 ParA family protein -
EXA01_RS23950 (30032) 30032..30340 + 309 WP_000030204.1 molecular chaperone GroEL -
EXA01_RS25675 (30562) 30562..30939 + 378 WP_000897156.1 transposase -
EXA01_RS23955 (30975) 30975..31855 + 881 Protein_45 IS66 family transposase -
EXA01_RS23960 (32033) 32033..33162 - 1130 Protein_46 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - cs3 / etpB / eltA / eltB 1..141411 141411


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T118787 WP_001159871.1 NZ_CP035847:28011-28316 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T118787 NZ_CP035847:28011-28316 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8440.51 Da        Isoelectric Point: 6.3212

>AT118787 WP_000829078.1 NZ_CP035847:27791-28009 [Escherichia coli]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT118787 NZ_CP035847:27791-28009 [Escherichia coli]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A7ZGP5

References