Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3621848..3622502 Replicon chromosome
Accession NZ_CP035846
Organism Escherichia coli strain TW10598

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EXA01_RS17615 Protein ID WP_000244781.1
Coordinates 3622095..3622502 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA01_RS17610 Protein ID WP_000354046.1
Coordinates 3621848..3622114 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA01_RS17590 (3617936) 3617936..3619369 - 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -
EXA01_RS17595 (3619414) 3619414..3619725 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EXA01_RS17600 (3619889) 3619889..3620548 + 660 WP_000250274.1 hemolysin III family protein -
EXA01_RS17605 (3620625) 3620625..3621605 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
EXA01_RS17610 (3621848) 3621848..3622114 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA01_RS17615 (3622095) 3622095..3622502 + 408 WP_000244781.1 protein YgfX Toxin
EXA01_RS17620 (3622542) 3622542..3623063 - 522 WP_001055874.1 flavodoxin FldB -
EXA01_RS17625 (3623175) 3623175..3624071 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
EXA01_RS17630 (3624096) 3624096..3624806 + 711 WP_000715224.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA01_RS17635 (3624812) 3624812..3626545 + 1734 WP_000813231.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T118777 WP_000244781.1 NZ_CP035846:3622095-3622502 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118777 NZ_CP035846:3622095-3622502 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118777 WP_000354046.1 NZ_CP035846:3621848-3622114 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118777 NZ_CP035846:3621848-3622114 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References