Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2215012..2215509 Replicon chromosome
Accession NZ_CP035846
Organism Escherichia coli strain TW10598

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EXA01_RS10820 Protein ID WP_001173343.1
Coordinates 2215012..2215185 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag EXA01_RS10825 Protein ID WP_071597404.1
Coordinates 2215213..2215509 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA01_RS10810 (2211677) 2211677..2213134 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
EXA01_RS10815 (2213371) 2213371..2214888 + 1518 WP_001295074.1 lysine--tRNA ligase -
EXA01_RS10820 (2215012) 2215012..2215185 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EXA01_RS10825 (2215213) 2215213..2215509 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EXA01_RS10830 (2215736) 2215736..2216008 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EXA01_RS10835 (2216020) 2216020..2216250 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
EXA01_RS10840 (2216431) 2216431..2218062 + 1632 WP_001216490.1 sensor histidine kinase -
EXA01_RS10845 (2218059) 2218059..2218778 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118772 WP_001173343.1 NZ_CP035846:c2215185-2215012 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118772 NZ_CP035846:c2215185-2215012 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT118772 WP_071597404.1 NZ_CP035846:c2215509-2215213 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118772 NZ_CP035846:c2215509-2215213 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTTCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References