Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 922432..924152 Replicon chromosome
Accession NZ_CP035846
Organism Escherichia coli strain TW10598

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag EXA01_RS04545 Protein ID WP_000312833.1
Coordinates 922432..923088 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID -
Locus tag EXA01_RS04550 Protein ID WP_000634201.1
Coordinates 923085..924152 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA01_RS04525 (917832) 917832..918086 - 255 WP_154670580.1 hypothetical protein -
EXA01_RS04530 (918368) 918368..918607 + 240 WP_001431807.1 hypothetical protein -
EXA01_RS04535 (918647) 918647..921085 + 2439 WP_000985465.1 type I restriction-modification system subunit M -
EXA01_RS04540 (921085) 921085..922419 + 1335 WP_000228013.1 restriction endonuclease subunit S -
EXA01_RS04545 (922432) 922432..923088 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
EXA01_RS04550 (923085) 923085..924152 + 1068 WP_000634201.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
EXA01_RS04555 (924171) 924171..927266 + 3096 WP_000108735.1 HsdR family type I site-specific deoxyribonuclease -
EXA01_RS04560 (927266) 927266..927982 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
EXA01_RS04565 (928301) 928301..928726 + 426 WP_000422743.1 transposase -
EXA01_RS04570 (928723) 928723..929073 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T118769 WP_000312833.1 NZ_CP035846:922432-923088 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

>T118769 NZ_CP035846:922432-923088 [Escherichia coli]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGCAAAAAGCTCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGAAACGTGGCAGCACACGACGTACCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGGATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40681.71 Da        Isoelectric Point: 5.8916

>AT118769 WP_000634201.1 NZ_CP035846:923085-924152 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
HHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp

>AT118769 NZ_CP035846:923085-924152 [Escherichia coli]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTGAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CACCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure

References