Detailed information of TA system TA118767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 357528..358166
• Replicon: chromosome
• Accession: NZ_CP035846
• Organism: Escherichia coli strain TW10598

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EXA01_RS01705
• Protein ID: WP_000813794.1
• Coordinates: 357990..358166 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EXA01_RS01700
• Protein ID: WP_001270286.1
• Coordinates: 357528..357944 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA01_RS01680 (352680)	352680..353621	-	942	WP_001251313.1	ABC transporter permease	-
EXA01_RS01685 (353622)	353622..354635	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
EXA01_RS01690 (354653)	354653..355798	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
EXA01_RS01695 (356043)	356043..357449	-	1407	WP_000760586.1	PLP-dependent aminotransferase family protein	-
EXA01_RS01700 (357528)	357528..357944	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EXA01_RS01705 (357990)	357990..358166	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EXA01_RS01710 (358388)	358388..358618	+	231	WP_000494244.1	YncJ family protein	-
EXA01_RS01715 (358710)	358710..360671	-	1962	WP_001355676.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EXA01_RS01720 (360744)	360744..361280	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
EXA01_RS01725 (361372)	361372..362547	+	1176	WP_001236251.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	362587..363852	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T118767 WP_000813794.1 NZ_CP035846:c358166-357990 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T118767 NZ_CP035846:c358166-357990 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT118767 WP_001270286.1 NZ_CP035846:c357944-357528 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT118767 NZ_CP035846:c357944-357528 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB