Detailed information of TA system TA118762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 68060..68703
• Replicon: plasmid pTW10722_147
• Accession: NZ_CP035842
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q2VNX7
• Locus tag: EXA02_RS26180
• Protein ID: WP_001044769.1
• Coordinates: 68287..68703 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: T2HRV4
• Locus tag: EXA02_RS26175
• Protein ID: WP_001261281.1
• Coordinates: 68060..68290 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS26150 (63799)	63799..64104	-	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	-
EXA02_RS26155 (64106)	64106..64324	-	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	-
EXA02_RS26160 (64879)	64879..65568	+	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
EXA02_RS26165 (65644)	65644..66756	+	1113	WP_024179954.1	IS4-like element IS421 family transposase	-
EXA02_RS26170 (66951)	66951..67640	-	690	WP_001433458.1	RES family NAD+ phosphorylase	-
EXA02_RS26175 (68060)	68060..68290	+	231	WP_001261281.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EXA02_RS26180 (68287)	68287..68703	+	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXA02_RS26185 (68879)	68879..69997	+	1119	Protein_95	IS3 family transposase	-
EXA02_RS28480 (70932)	70932..71705	+	774	WP_236923691.1	apolipoprotein acyltransferase	-
EXA02_RS26195 (71724)	71724..71873	+	150	WP_001399277.1	ATP-binding protein	-
EXA02_RS26200 (72267)	72267..72494	-	228	WP_001757586.1	bacterial lipid A biosynthesis acyltransferase family protein	-
EXA02_RS26205 (72746)	72746..73372	-	627	WP_000593827.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cssA / csfA	1..147979	147979
	-	inside	IScluster/Tn	-	-	52352..69906	17554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15183.63 Da
• Isoelectric Point: 7.7805

>T118762 WP_001044769.1 NZ_CP035842:68287-68703 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T118762 NZ_CP035842:68287-68703 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8769.89 Da
• Isoelectric Point: 4.3775

>AT118762 WP_001261281.1 NZ_CP035842:68060-68290 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPIRPTWGSFLECEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT118762 NZ_CP035842:68060-68290 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCATCCGGCCAACCTGGGGATCATTCCTAGAGTGCG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A7ZGW5

Antitoxin

• AlphaFold DB: T2HRV4