Detailed information of TA system TA118761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 63799..64324
• Replicon: plasmid pTW10722_147
• Accession: NZ_CP035842
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: T2HS38
• Locus tag: EXA02_RS26150
• Protein ID: WP_001159861.1
• Coordinates: 63799..64104 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: T2HUI2
• Locus tag: EXA02_RS26155
• Protein ID: WP_000813631.1
• Coordinates: 64106..64324 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS26120 (59424)	59424..59801	-	378	Protein_82	hypothetical protein	-
EXA02_RS26125 (59888)	59888..60910	+	1023	WP_001356151.1	IS21-like element IS100 family transposase	-
EXA02_RS26130 (60907)	60907..61689	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
EXA02_RS26135 (61911)	61911..62258	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
EXA02_RS26140 (62255)	62255..62635	-	381	WP_001466366.1	transposase	-
EXA02_RS26145 (62992)	62992..63798	-	807	WP_000099345.1	site-specific integrase	-
EXA02_RS26150 (63799)	63799..64104	-	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	Toxin
EXA02_RS26155 (64106)	64106..64324	-	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
EXA02_RS26160 (64879)	64879..65568	+	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
EXA02_RS26165 (65644)	65644..66756	+	1113	WP_024179954.1	IS4-like element IS421 family transposase	-
EXA02_RS26170 (66951)	66951..67640	-	690	WP_001433458.1	RES family NAD+ phosphorylase	-
EXA02_RS26175 (68060)	68060..68290	+	231	WP_001261281.1	type II toxin-antitoxin system VapB family antitoxin	-
EXA02_RS26180 (68287)	68287..68703	+	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cssA / csfA	1..147979	147979
	-	inside	IScluster/Tn	-	-	52352..69906	17554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11680.49 Da
• Isoelectric Point: 6.4674

>T118761 WP_001159861.1 NZ_CP035842:c64104-63799 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINMMFWGI

Nucleotide

• Length: 306 bp

>T118761 NZ_CP035842:c64104-63799 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTGCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGACTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATTGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAATGCCATTAACATGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT118761 WP_000813631.1 NZ_CP035842:c64324-64106 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMKNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT118761 NZ_CP035842:c64324-64106 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGAAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: T2HS38

Antitoxin

• AlphaFold DB: T2HUI2