Detailed information of TA system TA118760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 28851..29386
• Replicon: plasmid pTW10722_147
• Accession: NZ_CP035842
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T2HS21
• Locus tag: EXA02_RS25930
• Protein ID: WP_000222775.1
• Coordinates: 29099..29386 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T2HSA2
• Locus tag: EXA02_RS25925
• Protein ID: WP_001139207.1
• Coordinates: 28851..29102 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS25885 (24437)	24437..24649	+	213	WP_001301409.1	ANR family transcriptional regulator	-
EXA02_RS25890 (24895)	24895..25356	+	462	WP_001233850.1	thermonuclease family protein	-
EXA02_RS25895 (25402)	25402..25611	+	210	WP_001353639.1	Hha/YmoA family nucleoid-associated regulatory protein	-
EXA02_RS25900 (25649)	25649..26239	+	591	WP_000766827.1	DUF2726 domain-containing protein	-
EXA02_RS25905 (26479)	26479..26727	+	249	WP_000083840.1	replication regulatory protein RepA	-
EXA02_RS25910 (26972)	26972..27046	+	75	WP_001365705.1	RepA leader peptide Tap	-
EXA02_RS25915 (27039)	27039..27896	+	858	WP_000130987.1	incFII family plasmid replication initiator RepA	-
EXA02_RS25920 (28597)	28597..28764	+	168	WP_000841011.1	hypothetical protein	-
EXA02_RS25925 (28851)	28851..29102	+	252	WP_001139207.1	plasmid stabilization protein	Antitoxin
EXA02_RS25930 (29099)	29099..29386	+	288	WP_000222775.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXA02_RS25935 (29396)	29396..29926	+	531	WP_253207422.1	hypothetical protein	-
EXA02_RS25940 (30592)	30592..31194	-	603	WP_000517689.1	ProQ/FINO family protein	-
EXA02_RS25945 (31548)	31548..31796	+	249	Protein_46	hypothetical protein	-
EXA02_RS25950 (31835)	31835..33373	-	1539	WP_000998069.1	IS66 family transposase	-
EXA02_RS25955 (33423)	33423..33770	-	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
EXA02_RS25960 (33767)	33767..34147	-	381	WP_001171554.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cssA / csfA	1..147979	147979

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.17 Da
• Isoelectric Point: 10.6261

>T118760 WP_000222775.1 NZ_CP035842:29099-29386 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T118760 NZ_CP035842:29099-29386 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9216.45 Da
• Isoelectric Point: 4.0927

>AT118760 WP_001139207.1 NZ_CP035842:28851-29102 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT118760 NZ_CP035842:28851-29102 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: T2HS21

Antitoxin

• AlphaFold DB: A0A754EQ65