Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4403335..4404167 | Replicon | chromosome |
| Accession | NZ_CP035841 | ||
| Organism | Escherichia coli strain TW10722 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | - |
| Locus tag | EXA02_RS21425 | Protein ID | WP_000854762.1 |
| Coordinates | 4403335..4403709 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | S1NM52 |
| Locus tag | EXA02_RS21430 | Protein ID | WP_001295723.1 |
| Coordinates | 4403799..4404167 (-) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EXA02_RS21385 (4398755) | 4398755..4399021 | - | 267 | WP_001360325.1 | EutP/PduV family microcompartment system protein | - |
| EXA02_RS21390 (4399211) | 4399211..4399993 | - | 783 | WP_001667685.1 | IS21-like element IS100kyp family helper ATPase IstB | - |
| EXA02_RS21395 (4399987) | 4399987..4400961 | - | 975 | WP_001433436.1 | IS21-like element IS100 family transposase | - |
| EXA02_RS21400 (4401064) | 4401064..4401444 | + | 381 | WP_001171554.1 | transposase | - |
| EXA02_RS21405 (4401441) | 4401441..4401788 | + | 348 | WP_000612591.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| EXA02_RS21410 (4401838) | 4401838..4402899 | + | 1062 | Protein_4183 | IS66-like element ISEc8 family transposase | - |
| EXA02_RS21415 (4403006) | 4403006..4403086 | - | 81 | Protein_4184 | hypothetical protein | - |
| EXA02_RS21420 (4403186) | 4403186..4403338 | - | 153 | Protein_4185 | DUF5983 family protein | - |
| EXA02_RS21425 (4403335) | 4403335..4403709 | - | 375 | WP_000854762.1 | TA system toxin CbtA family protein | Toxin |
| EXA02_RS21430 (4403799) | 4403799..4404167 | - | 369 | WP_001295723.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| EXA02_RS21435 (4404265) | 4404265..4404699 | + | 435 | WP_001403014.1 | transposase | - |
| EXA02_RS21440 (4404696) | 4404696..4405046 | + | 351 | WP_000624688.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| EXA02_RS21445 (4405077) | 4405077..4406690 | + | 1614 | WP_001744949.1 | IS66-like element ISEc47 family transposase | - |
| EXA02_RS21450 (4406879) | 4406879..4407100 | - | 222 | WP_000692341.1 | DUF987 domain-containing protein | - |
| EXA02_RS21455 (4407163) | 4407163..4407639 | - | 477 | WP_001186774.1 | RadC family protein | - |
| EXA02_RS21460 (4407655) | 4407655..4408128 | - | 474 | WP_000855059.1 | antirestriction protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | IScluster/Tn | - | - | 4391765..4406690 | 14925 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14094.15 Da Isoelectric Point: 7.1881
>T118748 WP_000854762.1 NZ_CP035841:c4403709-4403335 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T118748 NZ_CP035841:c4403709-4403335 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13592.40 Da Isoelectric Point: 6.6255
>AT118748 WP_001295723.1 NZ_CP035841:c4404167-4403799 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK
Download Length: 369 bp
>AT118748 NZ_CP035841:c4404167-4403799 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|