Detailed information of TA system TA118746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3873499..3874124
• Replicon: chromosome
• Accession: NZ_CP035841
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXA02_RS18950
• Protein ID: WP_000911328.1
• Coordinates: 3873726..3874124 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EXA02_RS18945
• Protein ID: WP_000450524.1
• Coordinates: 3873499..3873726 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS18920 (3869302)	3869302..3869772	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EXA02_RS18925 (3869772)	3869772..3870344	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EXA02_RS18930 (3870490)	3870490..3871368	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EXA02_RS18935 (3871385)	3871385..3872419	+	1035	WP_001355860.1	outer membrane protein assembly factor BamC	-
EXA02_RS18940 (3872632)	3872632..3873345	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EXA02_RS18945 (3873499)	3873499..3873726	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EXA02_RS18950 (3873726)	3873726..3874124	+	399	WP_000911328.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EXA02_RS18955 (3874271)	3874271..3875134	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
EXA02_RS18960 (3875149)	3875149..3877164	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
EXA02_RS18965 (3877238)	3877238..3877936	+	699	WP_000679823.1	esterase	-
EXA02_RS18970 (3878046)	3878046..3878246	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14966.32 Da
• Isoelectric Point: 8.5325

>T118746 WP_000911328.1 NZ_CP035841:3873726-3874124 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYEAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T118746 NZ_CP035841:3873726-3874124 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGAGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT118746 WP_000450524.1 NZ_CP035841:3873499-3873726 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT118746 NZ_CP035841:3873499-3873726 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33