Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3363120..3363774 Replicon chromosome
Accession NZ_CP035841
Organism Escherichia coli strain TW10722

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EXA02_RS16425 Protein ID WP_000244781.1
Coordinates 3363367..3363774 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA02_RS16420 Protein ID WP_000354046.1
Coordinates 3363120..3363386 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA02_RS16395 (3358289) 3358289..3359032 + 744 WP_000951961.1 SDR family oxidoreductase -
EXA02_RS16400 (3359089) 3359089..3360522 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
EXA02_RS16405 (3360567) 3360567..3360878 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EXA02_RS16410 (3361042) 3361042..3361701 + 660 WP_000250274.1 hemolysin III family protein -
EXA02_RS16415 (3361897) 3361897..3362877 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
EXA02_RS16420 (3363120) 3363120..3363386 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA02_RS16425 (3363367) 3363367..3363774 + 408 WP_000244781.1 protein YgfX Toxin
EXA02_RS16430 (3363814) 3363814..3364335 - 522 WP_001055874.1 flavodoxin FldB -
EXA02_RS16435 (3364447) 3364447..3365343 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA02_RS16440 (3365368) 3365368..3366078 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA02_RS16445 (3366084) 3366084..3367817 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T118744 WP_000244781.1 NZ_CP035841:3363367-3363774 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118744 NZ_CP035841:3363367-3363774 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118744 WP_000354046.1 NZ_CP035841:3363120-3363386 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118744 NZ_CP035841:3363120-3363386 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References