Detailed information of TA system TA118743

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3251894..3252692
• Replicon: chromosome
• Accession: NZ_CP035841
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EXA02_RS15850
• Protein ID: WP_000854733.1
• Coordinates: 3251894..3252271 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EXA02_RS15855
• Protein ID: WP_032190265.1
• Coordinates: 3252318..3252692 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS15825 (3247786)	3247786..3248322	+	537	WP_000942799.1	GspM family type II secretion system protein YghD	-
EXA02_RS15830 (3248657)	3248657..3250192	-	1536	WP_000484448.1	EAL domain-containing protein	-
EXA02_RS15835 (3250263)	3250263..3251111	-	849	Protein_3091	DUF4942 domain-containing protein	-
EXA02_RS15840 (3251200)	3251200..3251397	-	198	WP_000839243.1	DUF957 domain-containing protein	-
EXA02_RS15845 (3251409)	3251409..3251897	-	489	WP_000761663.1	DUF5983 family protein	-
EXA02_RS15850 (3251894)	3251894..3252271	-	378	WP_000854733.1	TA system toxin CbtA family protein	Toxin
EXA02_RS15855 (3252318)	3252318..3252692	-	375	WP_032190265.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EXA02_RS15860 (3252742)	3252742..3253386	-	645	WP_000086771.1	hypothetical protein	-
EXA02_RS15865 (3253405)	3253405..3253626	-	222	WP_000692327.1	DUF987 domain-containing protein	-
EXA02_RS15870 (3253689)	3253689..3254165	-	477	WP_001502867.1	RadC family protein	-
EXA02_RS15875 (3254181)	3254181..3254654	-	474	WP_001355870.1	antirestriction protein	-
EXA02_RS15880 (3254723)	3254723..3254992	-	270	WP_001183319.1	hypothetical protein	-
EXA02_RS15885 (3254992)	3254992..3255810	-	819	WP_001234387.1	DUF932 domain-containing protein	-
EXA02_RS15890 (3256138)	3256138..3256449	-	312	Protein_3102	autotransporter adhesin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14236.21 Da
• Isoelectric Point: 6.8528

>T118743 WP_000854733.1 NZ_CP035841:c3252271-3251894 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNDITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T118743 NZ_CP035841:c3252271-3251894 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGTTGCCCGTCTCCCGTCACCATCTGGCAGACACTACTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13967.80 Da
• Isoelectric Point: 6.7388

>AT118743 WP_032190265.1 NZ_CP035841:c3252692-3252318 [Escherichia coli]
VSDKLHETNYPDDHNDRIWWGLPCTVTPCFGARLVQEGNRLHYLANRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADSLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT118743 NZ_CP035841:c3252692-3252318 [Escherichia coli]
GTGTCAGACAAGCTCCATGAGACAAATTATCCCGACGACCATAACGACCGCATCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCAACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACTCCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACTTCATAA