Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2735831..2736417 Replicon chromosome
Accession NZ_CP035841
Organism Escherichia coli strain TW10722

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag EXA02_RS13250 Protein ID WP_042014162.1
Coordinates 2735831..2736061 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EXA02_RS13255 Protein ID WP_000593555.1
Coordinates 2736058..2736417 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA02_RS13240 (2731868) 2731868..2734603 + 2736 WP_000149156.1 ribosome-associated ATPase/putative transporter RbbA -
EXA02_RS13245 (2734603) 2734603..2735727 + 1125 WP_001314210.1 ABC transporter permease -
EXA02_RS13250 (2735831) 2735831..2736061 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
EXA02_RS13255 (2736058) 2736058..2736417 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EXA02_RS13260 (2736537) 2736537..2736938 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
EXA02_RS13265 (2736944) 2736944..2737750 - 807 WP_000173667.1 nickel import ATP-binding protein NikE -
EXA02_RS13270 (2737747) 2737747..2738511 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
EXA02_RS13275 (2738511) 2738511..2739344 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
EXA02_RS13280 (2739341) 2739341..2740285 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T118740 WP_042014162.1 NZ_CP035841:2735831-2736061 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T118740 NZ_CP035841:2735831-2736061 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT118740 WP_000593555.1 NZ_CP035841:2736058-2736417 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT118740 NZ_CP035841:2736058-2736417 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References