Detailed information of TA system TA118731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1000411..1001248
• Replicon: chromosome
• Accession: NZ_CP035841
• Organism: Escherichia coli strain TW10722

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: EXA02_RS05040
• Protein ID: WP_000227784.1
• Coordinates: 1000706..1001248 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EXA02_RS05035
• Protein ID: WP_001297137.1
• Coordinates: 1000411..1000722 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA02_RS05010 (995431)	995431..996378	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
EXA02_RS05015 (996400)	996400..998391	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EXA02_RS05020 (998381)	998381..998995	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EXA02_RS05025 (998995)	998995..999324	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EXA02_RS05030 (999336)	999336..1000226	+	891	WP_000971336.1	heme o synthase	-
EXA02_RS05035 (1000411)	1000411..1000722	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EXA02_RS05040 (1000706)	1000706..1001248	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
EXA02_RS05045 (1001304)	1001304..1002239	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
EXA02_RS05050 (1002647)	1002647..1004011	+	1365	WP_001000978.1	MFS transporter	-
EXA02_RS05055 (1004139)	1004139..1004630	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
EXA02_RS05060 (1004798)	1004798..1005709	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T118731 WP_000227784.1 NZ_CP035841:1000706-1001248 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T118731 NZ_CP035841:1000706-1001248 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT118731 WP_001297137.1 NZ_CP035841:1000411-1000722 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT118731 NZ_CP035841:1000411-1000722 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3