Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 966333..966951 Replicon chromosome
Accession NZ_CP035841
Organism Escherichia coli strain TW10722

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXA02_RS04870 Protein ID WP_001291435.1
Coordinates 966733..966951 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXA02_RS04865 Protein ID WP_000344800.1
Coordinates 966333..966707 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA02_RS04855 (961422) 961422..962615 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EXA02_RS04860 (962638) 962638..965787 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXA02_RS04865 (966333) 966333..966707 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXA02_RS04870 (966733) 966733..966951 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EXA02_RS04875 (967123) 967123..967674 + 552 WP_000102568.1 maltose O-acetyltransferase -
EXA02_RS04880 (967790) 967790..968260 + 471 WP_000136192.1 YlaC family protein -
EXA02_RS04885 (968424) 968424..969974 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXA02_RS04890 (970016) 970016..970369 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EXA02_RS04900 (970748) 970748..971059 + 312 WP_000409911.1 MGMT family protein -
EXA02_RS04905 (971090) 971090..971662 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118730 WP_001291435.1 NZ_CP035841:966733-966951 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118730 NZ_CP035841:966733-966951 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118730 WP_000344800.1 NZ_CP035841:966333-966707 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118730 NZ_CP035841:966333-966707 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References