Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 57554..58113 Replicon plasmid pWS1933D_74
Accession NZ_CP035840
Organism Escherichia coli strain WS1933D

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag EXA03_RS26395 Protein ID WP_000421261.1
Coordinates 57554..57829 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag EXA03_RS26400 Protein ID WP_001297026.1
Coordinates 57829..58113 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA03_RS26365 (52842) 52842..53468 + 627 WP_000587689.1 ParA family plasmid-partitioning AAA ATPase -
EXA03_RS26370 (53465) 53465..53767 + 303 WP_001245884.1 hypothetical protein -
EXA03_RS26375 (54270) 54270..55616 - 1347 WP_001132022.1 protein YagA -
EXA03_RS26380 (55970) 55970..56572 + 603 WP_000517694.1 ProQ/FINO family protein -
EXA03_RS26385 (56710) 56710..56976 + 267 WP_000627831.1 hypothetical protein -
EXA03_RS26390 (57245) 57245..57448 - 204 Protein_65 hypothetical protein -
EXA03_RS26395 (57554) 57554..57829 - 276 WP_000421261.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EXA03_RS26400 (57829) 57829..58113 - 285 WP_001297026.1 ribbon-helix-helix domain-containing protein Antitoxin
EXA03_RS26405 (59021) 59021..60052 - 1032 WP_000907882.1 plasmid replication initiator RepA -
EXA03_RS26410 (60890) 60890..61396 + 507 WP_001317510.1 transcription termination/antitermination NusG family protein -
EXA03_RS26415 (61537) 61537..61947 + 411 WP_253208150.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..74294 74294


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T118727 WP_000421261.1 NZ_CP035840:c57829-57554 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEVRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T118727 NZ_CP035840:c57829-57554 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAGTCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10502.95 Da        Isoelectric Point: 5.6558

>AT118727 WP_001297026.1 NZ_CP035840:c58113-57829 [Escherichia coli]
MQMKNNTVQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT118727 NZ_CP035840:c58113-57829 [Escherichia coli]
ATGCAAATGAAAAACAATACCGTACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References