Detailed information of TA system TA118721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 120624..121183
• Replicon: plasmid pWS1933D_126
• Accession: NZ_CP035837
• Organism: Escherichia coli strain WS1933D

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: EXA03_RS24975
• Protein ID: WP_000421265.1
• Coordinates: 120908..121183 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: EXA03_RS24970
• Protein ID: WP_001743519.1
• Coordinates: 120624..120908 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA03_RS24950	116087..116869	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
EXA03_RS24955	116937..117377	+	441	Protein_124	recombinase family protein	-
EXA03_RS26715	117374..117787	+	414	WP_001046769.1	hypothetical protein	-
EXA03_RS24965	118119..119870	+	1752	WP_000038721.1	ATP-binding protein	-
EXA03_RS24970	120624..120908	+	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXA03_RS24975	120908..121183	+	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXA03_RS24980	121346..122422	+	1077	WP_000610004.1	hypothetical protein	-
EXA03_RS24985	122673..122876	+	204	Protein_130	hypothetical protein	-
EXA03_RS24995	123485..123559	-	75	Protein_131	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EXA03_RS25000	124973..125368	-	396	WP_000445942.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaD' / cfaE / cfaC / cfaB / cfaA / etpB / etpA / etpB / etpA	1..126275	126275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T118721 WP_000421265.1 NZ_CP035837:120908-121183 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118721 NZ_CP035837:120908-121183 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT118721 WP_001743519.1 NZ_CP035837:120624-120908 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118721 NZ_CP035837:120624-120908 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1