Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4516726..4517344 Replicon chromosome
Accession NZ_CP035836
Organism Escherichia coli strain WS1933D

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EXA03_RS22130 Protein ID WP_001291435.1
Coordinates 4516726..4516944 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EXA03_RS22135 Protein ID WP_000344800.1
Coordinates 4516970..4517344 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA03_RS22095 (4512015) 4512015..4512587 + 573 WP_000779826.1 YbaY family lipoprotein -
EXA03_RS22100 (4512618) 4512618..4512929 - 312 WP_000409911.1 MGMT family protein -
EXA03_RS22110 (4513308) 4513308..4513661 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EXA03_RS22115 (4513703) 4513703..4515253 - 1551 WP_001484361.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EXA03_RS22120 (4515417) 4515417..4515887 - 471 WP_000136192.1 YlaC family protein -
EXA03_RS22125 (4516003) 4516003..4516554 - 552 WP_000102568.1 maltose O-acetyltransferase -
EXA03_RS22130 (4516726) 4516726..4516944 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EXA03_RS22135 (4516970) 4516970..4517344 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EXA03_RS22140 (4517890) 4517890..4521039 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
EXA03_RS22145 (4521062) 4521062..4522255 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118720 WP_001291435.1 NZ_CP035836:c4516944-4516726 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118720 NZ_CP035836:c4516944-4516726 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118720 WP_000344800.1 NZ_CP035836:c4517344-4516970 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118720 NZ_CP035836:c4517344-4516970 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References