Detailed information of TA system TA118719

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4284109..4284788
• Replicon: chromosome
• Accession: NZ_CP035836
• Organism: Escherichia coli strain WS1933D

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: EXA03_RS20975
• Protein ID: WP_000854680.1
• Coordinates: 4284109..4284450 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: EXA03_RS20980
• Protein ID: WP_000070396.1
• Coordinates: 4284471..4284788 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA03_RS20960 (4280188)	4280188..4280535	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
EXA03_RS20965 (4280535)	4280535..4281212	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
EXA03_RS20970 (4281908)	4281908..4283014	+	1107	WP_000633074.1	SMEK domain-containing protein	-
EXA03_RS20975 (4284109)	4284109..4284450	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
EXA03_RS20980 (4284471)	4284471..4284788	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
EXA03_RS20985 (4284807)	4284807..4285028	-	222	WP_000691994.1	DUF987 domain-containing protein	-
EXA03_RS20990 (4285037)	4285037..4285513	-	477	WP_000811693.1	RadC family protein	-
EXA03_RS20995 (4285529)	4285529..4285987	-	459	WP_000211838.1	antirestriction protein	-
EXA03_RS21000 (4286085)	4286085..4286324	-	240	WP_000194654.1	DUF905 family protein	-
EXA03_RS21005 (4286401)	4286401..4286868	-	468	WP_001385283.1	protein YkfB	-
EXA03_RS21010 (4286891)	4286891..4287334	-	444	WP_000649865.1	hypothetical protein	-
EXA03_RS21015 (4287334)	4287334..4287570	-	237	WP_001144031.1	protein YpjK	-
EXA03_RS21020 (4287611)	4287611..4288312	-	702	WP_000189411.1	WYL domain-containing protein	-
EXA03_RS21025 (4288529)	4288529..4289350	-	822	WP_001385280.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T118719 WP_000854680.1 NZ_CP035836:c4284450-4284109 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T118719 NZ_CP035836:c4284450-4284109 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT118719 WP_000070396.1 NZ_CP035836:c4284788-4284471 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT118719 NZ_CP035836:c4284788-4284471 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7