Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3790069..3790664 Replicon chromosome
Accession NZ_CP035836
Organism Escherichia coli strain WS1933D

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag EXA03_RS18615 Protein ID WP_000239579.1
Coordinates 3790314..3790664 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag EXA03_RS18610 Protein ID WP_001223208.1
Coordinates 3790069..3790320 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA03_RS18600 (3785734) 3785734..3789513 + 3780 WP_000060907.1 autotransporter assembly complex protein TamB -
EXA03_RS18605 (3789516) 3789516..3789857 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EXA03_RS18610 (3790069) 3790069..3790320 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EXA03_RS18615 (3790314) 3790314..3790664 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
EXA03_RS18620 (3790741) 3790741..3791271 - 531 WP_000055072.1 inorganic diphosphatase -
EXA03_RS18625 (3791581) 3791581..3792537 + 957 WP_000265941.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EXA03_RS18630 (3792677) 3792677..3794179 + 1503 WP_000205800.1 sugar ABC transporter ATP-binding protein -
EXA03_RS18635 (3794193) 3794193..3795215 + 1023 WP_001295197.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T118718 WP_000239579.1 NZ_CP035836:3790314-3790664 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T118718 NZ_CP035836:3790314-3790664 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT118718 WP_001223208.1 NZ_CP035836:3790069-3790320 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT118718 NZ_CP035836:3790069-3790320 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References