Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2290100..2290754 Replicon chromosome
Accession NZ_CP035836
Organism Escherichia coli strain WS1933D

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EXA03_RS11400 Protein ID WP_000244781.1
Coordinates 2290100..2290507 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA03_RS11405 Protein ID WP_000354046.1
Coordinates 2290488..2290754 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA03_RS11380 (2286057) 2286057..2287790 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
EXA03_RS11385 (2287796) 2287796..2288506 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA03_RS11390 (2288531) 2288531..2289427 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA03_RS11395 (2289539) 2289539..2290060 + 522 WP_001055874.1 flavodoxin FldB -
EXA03_RS11400 (2290100) 2290100..2290507 - 408 WP_000244781.1 protein YgfX Toxin
EXA03_RS11405 (2290488) 2290488..2290754 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA03_RS11410 (2290997) 2290997..2291977 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
EXA03_RS11415 (2292173) 2292173..2292832 - 660 WP_000250274.1 hemolysin III family protein -
EXA03_RS11420 (2292996) 2292996..2293307 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EXA03_RS11425 (2293352) 2293352..2294785 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
EXA03_RS11430 (2294842) 2294842..2295585 - 744 WP_000951939.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T118714 WP_000244781.1 NZ_CP035836:c2290507-2290100 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118714 NZ_CP035836:c2290507-2290100 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118714 WP_000354046.1 NZ_CP035836:c2290754-2290488 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118714 NZ_CP035836:c2290754-2290488 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References