Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2088257..2089089 Replicon chromosome
Accession NZ_CP035836
Organism Escherichia coli strain WS1933D

Toxin (Protein)


Gene name yeeV Uniprot ID F4TJC5
Locus tag EXA03_RS10435 Protein ID WP_001094452.1
Coordinates 2088257..2088631 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7I8Z144
Locus tag EXA03_RS10440 Protein ID WP_016255729.1
Coordinates 2088721..2089089 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA03_RS10410 (2083646) 2083646..2085897 - 2252 Protein_2049 alpha-amylase family glycosyl hydrolase -
EXA03_RS10420 (2086631) 2086631..2087476 - 846 WP_001280506.1 DUF4942 domain-containing protein -
EXA03_RS10425 (2087561) 2087561..2087758 - 198 WP_000839264.1 DUF957 domain-containing protein -
EXA03_RS10430 (2087769) 2087769..2088260 - 492 WP_000977086.1 DUF5983 family protein -
EXA03_RS10435 (2088257) 2088257..2088631 - 375 WP_001094452.1 TA system toxin CbtA family protein Toxin
EXA03_RS10440 (2088721) 2088721..2089089 - 369 WP_016255729.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EXA03_RS10445 (2089164) 2089164..2089385 - 222 WP_000692315.1 DUF987 domain-containing protein -
EXA03_RS10450 (2089448) 2089448..2089924 - 477 WP_001484796.1 RadC family protein -
EXA03_RS10455 (2089939) 2089939..2090424 - 486 WP_000213708.1 antirestriction protein -
EXA03_RS10460 (2090516) 2090516..2091334 - 819 WP_001234594.1 DUF932 domain-containing protein -
EXA03_RS10465 (2091434) 2091434..2091667 - 234 WP_001117567.1 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14014.86 Da        Isoelectric Point: 7.2920

>T118712 WP_001094452.1 NZ_CP035836:c2088631-2088257 [Escherichia coli]
MNTLPDTHVREASRCSSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSTDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Download         Length: 375 bp

>T118712 NZ_CP035836:c2088631-2088257 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCTCATCTCCCGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCACCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13750.53 Da        Isoelectric Point: 6.7395

>AT118712 WP_016255729.1 NZ_CP035836:c2089089-2088721 [Escherichia coli]
VSDTLHETTHPDDNRGRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT118712 NZ_CP035836:c2089089-2088721 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAACTCATCCCGACGACAATCGCGGCCGCCCCTGGTGGGGACTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGA
GAACTGAATCCCCGTCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7YIM6

Antitoxin

Source ID Structure
AlphaFold DB A0A7I8Z144

References