Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/RelE-RHH |
| Location | 54846..55393 | Replicon | plasmid p025542_72 |
| Accession | NZ_CP035835 | ||
| Organism | Escherichia coli strain 025542-1 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | G7QEJ9 |
| Locus tag | EXA04_RS26165 | Protein ID | WP_001384452.1 |
| Coordinates | 54846..55124 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | Q4FIL6 |
| Locus tag | EXA04_RS26170 | Protein ID | WP_000079945.1 |
| Coordinates | 55124..55393 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EXA04_RS26130 (50121) | 50121..50432 | - | 312 | WP_032144809.1 | hypothetical protein | - |
| EXA04_RS26135 (50429) | 50429..51331 | - | 903 | WP_001513647.1 | DUF1281 domain-containing protein | - |
| EXA04_RS26565 (51426) | 51426..51695 | - | 270 | WP_072126982.1 | hypothetical protein | - |
| EXA04_RS26140 (51866) | 51866..52828 | + | 963 | WP_000959884.1 | plasmid segregation protein ParM | - |
| EXA04_RS26145 (52831) | 52831..53181 | + | 351 | WP_001414994.1 | plasmid partitioning/stability family protein | - |
| EXA04_RS26150 (53290) | 53290..53709 | + | 420 | WP_024181414.1 | hypothetical protein | - |
| EXA04_RS26155 (53644) | 53644..54525 | - | 882 | WP_000005460.1 | hypothetical protein | - |
| EXA04_RS26160 (54596) | 54596..54774 | - | 179 | Protein_80 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EXA04_RS26165 (54846) | 54846..55124 | - | 279 | WP_001384452.1 | type II toxin-antitoxin system toxin YacB | Toxin |
| EXA04_RS26170 (55124) | 55124..55393 | - | 270 | WP_000079945.1 | type II toxin-antitoxin system antitoxin YacA | Antitoxin |
| EXA04_RS26175 (55466) | 55466..55606 | - | 141 | WP_000829596.1 | hypothetical protein | - |
| EXA04_RS26180 (56309) | 56309..57166 | - | 858 | WP_000130995.1 | incFII family plasmid replication initiator RepA | - |
| EXA04_RS26570 (57185) | 57185..57365 | - | 181 | Protein_85 | protein CopA/IncA | - |
| EXA04_RS26190 (57480) | 57480..57728 | - | 249 | WP_000083839.1 | replication regulatory protein RepA | - |
| EXA04_RS26195 (57968) | 57968..58558 | - | 591 | WP_000766807.1 | DUF2726 domain-containing protein | - |
| EXA04_RS26200 (58596) | 58596..58805 | - | 210 | WP_001298565.1 | hemolysin expression modulator Hha | - |
| EXA04_RS26205 (58851) | 58851..59312 | - | 462 | WP_000978821.1 | thermonuclease family protein | - |
| EXA04_RS26210 (59556) | 59556..59747 | - | 192 | WP_071528006.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(B) | - | 1..72087 | 72087 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10762.38 Da Isoelectric Point: 8.4615
>T118700 WP_001384452.1 NZ_CP035835:c55124-54846 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
Download Length: 279 bp
>T118700 NZ_CP035835:c55124-54846 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 10180.52 Da Isoelectric Point: 8.8735
>AT118700 WP_000079945.1 NZ_CP035835:c55393-55124 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK
Download Length: 270 bp
>AT118700 NZ_CP035835:c55393-55124 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTTCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTTCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CL36 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q4FIL6 |