Detailed information of TA system TA118699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 58253..58812
• Replicon: plasmid p025542_98
• Accession: NZ_CP035833
• Organism: Escherichia coli strain 025542-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: EXA04_RS25070
• Protein ID: WP_000421265.1
• Coordinates: 58537..58812 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: EXA04_RS25065
• Protein ID: WP_001743519.1
• Coordinates: 58253..58537 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA04_RS25045	53665..54231	+	567	WP_000904908.1	recombinase family protein	-
EXA04_RS26510	54228..54641	+	414	WP_001046769.1	hypothetical protein	-
EXA04_RS25055	55090..55787	+	698	WP_099550739.1	IS1 family transposase	-
EXA04_RS26515	55807..56553	+	747	WP_235838502.1	ATP-binding protein	-
EXA04_RS26520	56870..57499	+	630	WP_000943192.1	hypothetical protein	-
EXA04_RS25065	58253..58537	+	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
EXA04_RS25070	58537..58812	+	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXA04_RS25075	58975..60051	+	1077	WP_000610005.1	hypothetical protein	-
EXA04_RS26525	60302..60382	+	81	Protein_63	hypothetical protein	-
EXA04_RS25080	60381..61287	-	907	Protein_64	IS3-like element ISEc52 family transposase	-
EXA04_RS25085	61297..62448	-	1152	WP_001254932.1	IS30-like element IS30 family transposase	-
EXA04_RS25090	62516..62773	-	258	Protein_66	transposase	-
EXA04_RS26530	62844..62981	+	138	Protein_67	hypothetical protein	-
EXA04_RS25105	63590..63664	-	75	Protein_68	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	etpB / etpA / cfaD' / cfaE / cfaC / cfaB / cfaA	1..98355	98355
	-	inside	IScluster/Tn	-	-	50486..62773	12287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T118699 WP_000421265.1 NZ_CP035833:58537-58812 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118699 NZ_CP035833:58537-58812 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT118699 WP_001743519.1 NZ_CP035833:58253-58537 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118699 NZ_CP035833:58253-58537 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1