Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4653551..4654048 Replicon chromosome
Accession NZ_CP035832
Organism Escherichia coli strain 025542-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EXA04_RS22705 Protein ID WP_001173343.1
Coordinates 4653551..4653724 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EXA04_RS22710 Protein ID WP_000398619.1
Coordinates 4653752..4654048 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA04_RS22695 (4650221) 4650221..4651678 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
EXA04_RS22700 (4651915) 4651915..4653432 + 1518 WP_001295074.1 lysine--tRNA ligase -
EXA04_RS22705 (4653551) 4653551..4653724 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EXA04_RS22710 (4653752) 4653752..4654048 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EXA04_RS22715 (4654275) 4654275..4654547 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EXA04_RS22720 (4654559) 4654559..4654789 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
EXA04_RS22725 (4654970) 4654970..4656601 + 1632 WP_001216486.1 sensor histidine kinase -
EXA04_RS22730 (4656598) 4656598..4657317 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118697 WP_001173343.1 NZ_CP035832:c4653724-4653551 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118697 NZ_CP035832:c4653724-4653551 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT118697 WP_000398619.1 NZ_CP035832:c4654048-4653752 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118697 NZ_CP035832:c4654048-4653752 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References