Detailed information of TA system TA118694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3916939..3917776
• Replicon: chromosome
• Accession: NZ_CP035832
• Organism: Escherichia coli strain 025542-1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: EXA04_RS19255
• Protein ID: WP_000227784.1
• Coordinates: 3917234..3917776 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EXA04_RS19250
• Protein ID: WP_001297137.1
• Coordinates: 3916939..3917250 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA04_RS19225 (3911959)	3911959..3912906	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
EXA04_RS19230 (3912928)	3912928..3914919	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EXA04_RS19235 (3914909)	3914909..3915523	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EXA04_RS19240 (3915523)	3915523..3915852	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EXA04_RS19245 (3915864)	3915864..3916754	+	891	WP_000971336.1	heme o synthase	-
EXA04_RS19250 (3916939)	3916939..3917250	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EXA04_RS19255 (3917234)	3917234..3917776	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
EXA04_RS19260 (3917832)	3917832..3918767	-	936	WP_001387350.1	tetratricopeptide repeat protein	-
EXA04_RS19265 (3919175)	3919175..3920539	+	1365	WP_001000965.1	MFS transporter	-
EXA04_RS19270 (3920667)	3920667..3921158	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
EXA04_RS19275 (3921326)	3921326..3922237	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T118694 WP_000227784.1 NZ_CP035832:3917234-3917776 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T118694 NZ_CP035832:3917234-3917776 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT118694 WP_001297137.1 NZ_CP035832:3916939-3917250 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT118694 NZ_CP035832:3916939-3917250 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3