Detailed information of TA system TA118682

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2001101..2001933
• Replicon: chromosome
• Accession: NZ_CP035832
• Organism: Escherichia coli strain 025542-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EXA04_RS09660
• Protein ID: WP_000854762.1
• Coordinates: 2001101..2001475 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: EXA04_RS09665
• Protein ID: WP_001295723.1
• Coordinates: 2001565..2001933 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA04_RS09635 (1996317)	1996317..1997825	-	1509	WP_001189123.1	group II intron reverse transcriptase/maturase	-
EXA04_RS09640 (1998498)	1998498..1999237	-	740	Protein_1888	IS3 family transposase	-
EXA04_RS09645 (1999287)	1999287..2000651	+	1365	Protein_1889	IS66-like element ISEc23 family transposase	-
EXA04_RS09650 (2000772)	2000772..2000852	-	81	Protein_1890	hypothetical protein	-
EXA04_RS09655 (2000952)	2000952..2001104	-	153	Protein_1891	DUF5983 family protein	-
EXA04_RS09660 (2001101)	2001101..2001475	-	375	WP_000854762.1	TA system toxin CbtA family protein	Toxin
EXA04_RS09665 (2001565)	2001565..2001933	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EXA04_RS09670 (2002096)	2002096..2002317	-	222	WP_000692341.1	DUF987 domain-containing protein	-
EXA04_RS09675 (2002380)	2002380..2002856	-	477	WP_001186774.1	RadC family protein	-
EXA04_RS09680 (2002872)	2002872..2003345	-	474	WP_000855059.1	antirestriction protein	-
EXA04_RS09685 (2003687)	2003687..2004505	-	819	WP_001234652.1	DUF932 domain-containing protein	-
EXA04_RS09690 (2004623)	2004623..2004818	-	196	Protein_1898	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14094.15 Da
• Isoelectric Point: 7.1881

>T118682 WP_000854762.1 NZ_CP035832:c2001475-2001101 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T118682 NZ_CP035832:c2001475-2001101 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT118682 WP_001295723.1 NZ_CP035832:c2001933-2001565 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118682 NZ_CP035832:c2001933-2001565 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52