Detailed information of TA system TA118680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1473594..1474219
• Replicon: chromosome
• Accession: NZ_CP035832
• Organism: Escherichia coli strain 025542-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U9YZ02
• Locus tag: EXA04_RS07225
• Protein ID: WP_000911329.1
• Coordinates: 1473821..1474219 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EXA04_RS07220
• Protein ID: WP_001696518.1
• Coordinates: 1473594..1473821 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA04_RS07195 (1469396)	1469396..1469866	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EXA04_RS07200 (1469866)	1469866..1470438	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EXA04_RS07205 (1470584)	1470584..1471462	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EXA04_RS07210 (1471479)	1471479..1472513	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EXA04_RS07215 (1472726)	1472726..1473439	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EXA04_RS07220 (1473594)	1473594..1473821	+	228	WP_001696518.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EXA04_RS07225 (1473821)	1473821..1474219	+	399	WP_000911329.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXA04_RS07230 (1474366)	1474366..1475229	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
EXA04_RS07235 (1475244)	1475244..1477259	+	2016	WP_001388162.1	tRNA cytosine(34) acetyltransferase TmcA	-
EXA04_RS07240 (1477333)	1477333..1478031	+	699	WP_000679812.1	esterase	-
EXA04_RS07245 (1478112)	1478112..1478312	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algU	1332691..1502384	169693

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.23 Da
• Isoelectric Point: 8.5325

>T118680 WP_000911329.1 NZ_CP035832:1473821-1474219 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T118680 NZ_CP035832:1473821-1474219 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8526.66 Da
• Isoelectric Point: 4.3867

>AT118680 WP_001696518.1 NZ_CP035832:1473594-1473821 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIVIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT118680 NZ_CP035832:1473594-1473821 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGTGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0XW84