Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 965149..965803 Replicon chromosome
Accession NZ_CP035832
Organism Escherichia coli strain 025542-1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EXA04_RS04715 Protein ID WP_000244772.1
Coordinates 965396..965803 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EXA04_RS04710 Protein ID WP_000354046.1
Coordinates 965149..965415 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA04_RS04685 (960437) 960437..961180 + 744 WP_000951964.1 SDR family oxidoreductase -
EXA04_RS04690 (961237) 961237..962670 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
EXA04_RS04695 (962715) 962715..963026 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EXA04_RS04700 (963190) 963190..963849 + 660 WP_000250274.1 hemolysin III family protein -
EXA04_RS04705 (963926) 963926..964906 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EXA04_RS04710 (965149) 965149..965415 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EXA04_RS04715 (965396) 965396..965803 + 408 WP_000244772.1 protein YgfX Toxin
EXA04_RS04720 (965843) 965843..966364 - 522 WP_001055874.1 flavodoxin FldB -
EXA04_RS04725 (966476) 966476..967372 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EXA04_RS04730 (967397) 967397..968107 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EXA04_RS04735 (968113) 968113..969846 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T118678 WP_000244772.1 NZ_CP035832:965396-965803 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118678 NZ_CP035832:965396-965803 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118678 WP_000354046.1 NZ_CP035832:965149-965415 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118678 NZ_CP035832:965149-965415 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References