Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 369238..369869 Replicon chromosome
Accession NZ_CP035832
Organism Escherichia coli strain 025542-1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag EXA04_RS01700 Protein ID WP_001259385.1
Coordinates 369238..369513 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag EXA04_RS01705 Protein ID WP_000593554.1
Coordinates 369510..369869 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA04_RS01685 (364242) 364242..365309 + 1068 WP_000361467.1 HlyD family secretion protein -
EXA04_RS01690 (365306) 365306..368041 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
EXA04_RS01695 (368041) 368041..369165 + 1125 WP_001314210.1 ABC transporter permease -
EXA04_RS01700 (369238) 369238..369513 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
EXA04_RS01705 (369510) 369510..369869 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EXA04_RS01710 (369919) 369919..370779 - 861 WP_001131783.1 class II aldolase -
EXA04_RS01715 (370811) 370811..371080 - 270 WP_000933060.1 HPr family phosphocarrier protein -
EXA04_RS01720 (371070) 371070..372578 - 1509 WP_001298718.1 FGGY-family carbohydrate kinase -
EXA04_RS01725 (372571) 372571..373929 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
EXA04_RS01730 (374006) 374006..374287 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
EXA04_RS01735 (374284) 374284..374757 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 351469..369869 18400


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T118676 WP_001259385.1 NZ_CP035832:369238-369513 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T118676 NZ_CP035832:369238-369513 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT118676 WP_000593554.1 NZ_CP035832:369510-369869 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT118676 NZ_CP035832:369510-369869 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References