Detailed information of TA system TA118669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 23844..24469
• Replicon: plasmid p67_83
• Accession: NZ_CP035829
• Organism: Escherichia coli strain 67-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXA05_RS25825
• Protein ID: WP_000911313.1
• Coordinates: 24071..24469 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: EXA05_RS25820
• Protein ID: WP_000450520.1
• Coordinates: 23844..24071 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA05_RS25820 (23844)	23844..24071	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EXA05_RS25825 (24071)	24071..24469	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXA05_RS25830 (24478)	24478..26511	-	2034	Protein_29	type IV conjugative transfer system coupling protein TraD	-
EXA05_RS25835 (26543)	26543..28114	-	1572	WP_001748001.1	IS66-like element ISCro1 family transposase	-
EXA05_RS25840 (28134)	28134..28481	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
EXA05_RS25845 (28481)	28481..29158	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..83544	83544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T118669 WP_000911313.1 NZ_CP035829:24071-24469 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T118669 NZ_CP035829:24071-24469 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT118669 WP_000450520.1 NZ_CP035829:23844-24071 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT118669 NZ_CP035829:23844-24071 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2